|Powers= Can kill most [[demon]]s and [[hellhound]]s.
|location= Currently in [[Sam]] and [[Dean]]'s possession.
|episodes= '''First Appearance:''' [[3.01 The Magnificent Seven]]<br>'''Last Appearance:''' [[9.11 First Born14 Captives]]
}}
When four demons attack [[Cain]]'s house, Dean takes on three with the knife while Crowley kills the last with an [[angel sword]]. Despite an intense fight in which Dean briefly loses the knife to one of the demons, he manages to kill all three single-handedly, proving himself to Cain to be worthy of the [[Mark of Cain]]. When Cain refuses to give Dean the First Blade, Dean threatens him with the knife, but Cain merely stabs himself with it, proving to be completely immune to the knife's powers with it not even hurting him.
===[[9.14 Captives]]===
==Notes==