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There is speculation about what other powerful beings may not be killed by the Colt including:
* [[God]]/[[Chuck]] and [[Amara]] – Given their status as the cosmic
embodiments of light and darkness, it is likely they are invulnerable.* [[Archangels]] - Lucifer is shown to be invulnerable to the Colt, but a head shot disorients him for a few seconds . Since [[Michael]] is more powerful, it stands to reason that he cannot be harmed by the Colt either. Michael and Lucifer are on different power levels than [[Gabriel]] and [[Raphael]], implying that they may be vulnerable to the Colt.
* [[Four Horsemen of the Apocalypse]]: [[Famine]], [[Pestilence]] and [[War]] were rendered powerless with the removal of their rings, so would possibly be vulnerable to the Colt. [[Death]] maintains its power even without its ring, and seems to be more powerful than the other Horsemen. Given Death's claim it would eventually reap God, it may be invulnerable to the Colt.
* [[Cain]] – When bearing the [[Mark of Cain]] which was given to him by [[Lucifer]], this may have protected him from the Colt. However, he was a [[Knight of Hell]], lower in the hierarchy than the [[Princes of Hell]] who could be killed by the Colt, implying that the Colt can kill Cain.* [[Eve]] –
The Colt is capable of killing the Alphas, her 'children', which implies that the Colt is capable of killing her.