[[Samuel]] and his people capture the Alpha Vampire. They subdue him using an I.V. of [[Dead Man's Blood (Pad of Definitions)|Dead Man's Blood]] and attempt to interrogate him, but he is extremely resistant to pain and gives Samuel no information. After Samuel leaves the room where he is being held, the alpha is approached by Dean and Sam. He reveals to them that, though he is the first vampire, he has a mother. He recognizes Dean and he seems interested in Sam's soullessness. He also tells them that Samuel is torturing him for information about [[Purgatory]] and where it is located. Shortly after their conversation, he breaks out of his cage and almost escapes, but demons appear and take him away on [[Crowley|Crowley's]] orders. It is revealed that Samuel has been working for Crowley, who will continue to question the Alpha Vampire about the location of Purgatory.
===[[6.08 All Dogs Go To to Heaven]]===
The Alpha Vampire does not appear, but is briefly mentioned when Crowley asks Sam and Dean to find another Alpha. Sam and Dean ask if the Alpha Vampire wasn't enough for him. It is possible that Crowley could get no information from him and killed him.