Dean reveals to Sam the "big secret" John told him in 2.01 In My Time of Dying - that Dean must be ready to kill Sam if Sam becomes evil. Sam is enraged and tries to convince Dean that they have to track down the rest of the Special Children to find out more about what's going on. Dean is reluctant, however, and is more inclined to go to ground instead. He begs Sam to lay low with him for a while, but later on Sam sneaks out of their motel room, steals a car, and leaves alone.
At the Roadhouse, Sam gets Ash to track down a list of children whose mothers died in nursery fires in 1983. Ash comes up with a short list: Sam, Max Miller, Andy Gallagher, and Scott Carey. Scott's murder by an unknown assailant formed the opening sequence of the episode. Sam decides to investigate. While talking with Ellen, Sam also finds out that Jo has run away and is hunting on her own.
While staying in the town where Scott was murdered, Sam meets Ava Wilson, who has tracked him down after having a vision of Sam's death (which we see her experience earlier in the episode). Ava reveals she also had a premonition of Scott's death. While they are talking in the motel room, Gordon fires upon them from a nearby rooftop.
Dean, who has tracked Sam down with Ellen's help, attacks Gordon in order to stop him but Gordon wins the fight, knocking Dean unconscious and abducting him. Holding Dean hostage, Gordon uses him as bait to lure Sam into an abandoned house he has booby-trapped with grenades. This is the setting and situation of Ava's vision. Gordon reveals to Dean that a demon during an exorcism spoke of a coming war and named Sam as a soldier in the demon army, and that this is his motivation - he believes Sam is evil (or will undoubtedly become so), and thus needs to be killed. He also cites an "inside source" at the Roadhouse leading him to Sam's location.
Ava tries to convince Sam to take her with him to the abandoned house but Sam convinces her to go home instead. Sam manages to avoid the traps and fights with Gordon, knocking him unconscious and escaping with Dean. As they are leaving the house, Gordon chases them, but is stopped when several police cars pull up (a situation of Sam's engineering). The police discover his weapons stash and arrest him.
Unable to reach Ava on the phone, Sam and Dean proceed to Ava's house where they discover her fiance with his throat cut, Ava's engagement ring in a pool of blood on the floor, and traces of sulfur on the window sill. Ava is nowhere to be found.
Before Dad died he, he told me something — something about you.
Sam: What? Dean, what did he tell you?
Dean: He said that he wanted me to watch out for you, to take care of you.
Sam: He told you that a million times.
Dean: No, this time was different. He said that I had to save you.
Sam: Save me from what?
Dean: He just said that I had to save you, that nothing else mattered; and that if I couldn't, I'd...
Sam: You'd what, Dean?
That I'd have to kill you. He said that I might have to kill you, Sammy.
Sam: That was fast.
Ash: Well, apparently, that's my job. Make the monkey dance at the keyboard.
Ava: Okay. you know what? Screw you, buddy. Okay? Because I'm a secretary from Peoria and I'm not part of anything! Okay? Do you see this? I am getting married in eight weeks. I am supposed to be at home addressing invitations, which I am way behind on, by the way. But instead, I drove out here to save your weirdo ass. But if you just want to stay here and die, fine. Me? I'm due back on planet Earth.
Are you okay?
Ava: Am I okay?
I just helped you steal some dead guy's confidential psych files. I'm awesome!
Waxler: What else does the yellow-eyed man say?
Scott: He has plans for me. He says there's a war coming. That people like me, we're going to be the soldiers. Everything's about to change.
So Gordy. I know me and Sam ain't exactly your favorite people, but don't you think this is a little extreme?
Gordon: What, you think this is revenge?
Dean: Well, we did leave you tied up in your own mess for three days. Which was awesome. Sorry, I shouldn't laugh.
Gordon: Yeah. I was definitely planning on whuppin' your ass for that.
But that's not what this is. This isn't personal. I'm not a killer, Dean. I'm a hunter. And your brother's fair game.
See, I was doing an exorcism down in Louisiana. Teenage girl, seemed routine, some low-level demon. But between all the jabbering and the head-spinning, the damn thing muttered something. About a coming war. And I don't think it meant to, it just kind of slipped out. But it was too late. Piqued my interest. And you can really make a demon talk, you got the right tools.
Dean: And what happened to the girl it was possessing?
Gordon: She didn't make it.
Dean: Well, you're a son of a bitch.
That's my momma you're talking about... Anyway. This demon tells me there are soldiers to fight in this coming war. Humans, fighting on hell's side. You believe that? I mean, they're psychics, so they're not exactly pure humans, but still. What kind of worthless scumbag have you got to be to turn against your own race? But you know the biggest kick in the ass? This demon said I knew one of them. Our very own Sammy Winchester.
Dean: Well, Gordon should be reaching for the soap for the next few years at least.
Sam: Yeah. If they pin Scott Carey's murder on him. And if he doesn't bust out.
Screw the job. Screw it, man, I'm sick of the job anyway. I mean, we don't get paid, we don't get thanked. The only thing we get's bad luck.
Sam: Well, come on, dude, you're a hunter. I mean, it's what you were meant to do.
Ah, I wasn't meant to do anything, I don't believe in that destiny crap.
Am I supposed to go dark side or something?
- To go dark side is a Star Wars reference that means to become evil.
These are .223 Caliber, subsonic rounds, the guy must've put a suppressor on the rifle.
Ava: Dude, who are you?
Sam: I ah...I just I... I just watch a lot of TJ Hooker.
- TJ Hooker was a 1980s cop show, that starred William Shatner.
Yeah, I talked to Ellen. Just got here myself. It's a real funky town. You ditched me, Sammy.
- "Funkytown" was a 1980 song by the disco band Lipps Inc. and was one of the last big disco hits.
Besides Mr. Tinkles the cat, no.
- Mr. Tinkles is a cat from the movie Cats and Dogs.
I'm surprised at you, Dean. Getting all emotional. I'd heard you were more of a professional than this. Look, let's say you were cruising around in that car of yours and, uh, you had little Hitler riding shotgun, right? Back when he was just some goofy, crappy artist. But you knew what he was going to turn into someday. You'd take him out, no questions, am I right?
- Referencing the fact that Adolf Hitler was a art student prior to entering politics.
One word: Amsterdam. Come on, man, I hear the coffeeshops don't even serve coffee.
- Referencing the fact that coffeeshops in Amsterdam often sell cannabis