|Episode(s)|| 6.05 Live Free or Twihard|
6.07 Family Matters
7.22 There Will Be Blood
The Alpha Vampire was the very first Vampire and progenitor of all other vampires. He was one of the Alpha monsters captured and interrogated by Crowley and Castiel in their efforts to locate Purgatory.
Powers and Abilities
- Resistant to pain, which it demonstrates when Samuel and Dean electrocute it
- Capable of superhuman feats, even exceeding those demonstrated by normal Vampires
- Can communicate telepathically with other vampires 
The Alpha Vampire is first glimpsed briefly flashing in the minds of its "children" as it commands them to recruit more for its army. Nothing is known about this particular monster, aside from the fact its messages can be heard by all vampires and render them unconscious. Dean sees this vision while he is infected:
A man sitting on a bench in the night. A little girl in a frilly dress and a flower in her hair. A graveyard filled with white wooden crosses. A cell dividing. The alpha vampire reaching out, with long, sharp fingernails. A large, ivy-covered house. The girl again. The alpha vamp on the bench. Blood cells rushing through a vein. The graveyard, dissolving into a classroom with rows upon rows of empty desks. The little girl. The alpha vamp by a pond. The dividing cell. A computer animation of the circulatory system, dissolving into a roadmap, where the highways pump like veins. A red circle is drawn around Aurora, Illinois. The alpha vampire by the pond.
The graveyard, then back to the alpha vampire, then the graveyard. The ivy-covered house, where the little girl is having a tea party on the lawn -- at night. She feeds her dolls blood.
The graveyard. A pulsating cell. Blood in the vein. The cell divides. Another shot of the cell dividing. The little girl is standing under a tree. Suddenly a twin flickers in beside her. She smiles, revealing a mouth full of fangs. The twin does the same. The doll with blood on her face. The alpha vamp on the bench, only now the twin girls are sitting on the ground next to the bench. The dividing cell. A weather vane with a bird. The alpha vamp at the pond. The twin girls play with their dolls next to the pond; they look up at the alpha vampire. He bends over and strokes one of the twins' faces with his pointed fingernail. It leaves a trail of blood on her cheek. He smiles fondly. Blood cells in the vein. The bloody doll. The graveyard. The alpha vamp and the twins; he straightens up and points. The front hallway of the house; the door opens, and the twins walk through, hand in hand. A doll's hand. One twin. The house. The alpha vamp reaching. A cell dividing. The graveyard. One twin. The alpha vampire on the bench.
Samuel and his people capture the Alpha Vampire. They subdue him using an I.V. of Dead Man's Blood and attempt to interrogate him, but he is extremely resistant to pain and gives Samuel no information. After Samuel leaves the room where he is being held, the alpha is approached by Dean and Sam. He reveals to them that, though he is the first vampire, he has a mother. He recognizes Dean and he seems interested in Sam's soullessness. He also tells them that Samuel is torturing him for information about Purgatory and where it is located. Shortly after their conversation, he breaks out of his cage and almost escapes, but demons appear and take him away on Crowley's orders. It is revealed that Samuel has been working for Crowley, who will continue to question the Alpha Vampire about the location of Purgatory.
The Alpha Vampire does not appear, but is briefly mentioned when Crowley asks Sam and Dean to find another Alpha. Sam and Dean ask if the Alpha Vampire wasn't enough for him. It is possible that Crowley could get no information from him and killed him.
Sam and Dean visits Alpha Vampire in his mansion to get his blood, but he is few steps ahead of them and lures them into a trap. He's not concerned by the brothers telling him that his life is in danger and that the Leviathan are not his friends, so he lockes them in another room. Short time after, he is visited by Edgar, whose orders are to prevent the Winchesters from getting Alpha's blood. When things go sideways and Edgar tries to kill the Alpha, Sam and Dean chop off the Leviathan's head. In the end brothers receive the blood along with a kidnapped boy.