|Episode(s)|| 6.05 Live Free or Twihard|
6.07 Family Matters
6.08 All Dogs Go to Heaven (mentioned)
7.22 There Will Be Blood
The Alpha Vampire was the very first vampire and progenitor of all other vampires. He was one of the Alpha monsters captured and interrogated by Crowley and Castiel in their efforts to locate Purgatory.
Powers and Abilities
- Resistant to pain, which it demonstrates when Samuel and Dean electrocute it.
- Capable of superhuman feats, even exceeding those demonstrated by normal vampires.
- Can communicate telepathically with other vampires. 
- Retractable claws.
The Alpha Vampire is first glimpsed briefly flashing in the minds of its "children" as it commands them to recruit more for its army. Nothing is known about this particular monster, aside from the fact its messages can be heard by all vampires and render them unconscious. Dean sees this vision while he is infected:
A man sitting on a bench in the night. A little girl in a frilly dress and a flower in her hair. A graveyard filled with white wooden crosses. A cell dividing. The Alpha Vampire reaching out, with long, sharp fingernails. A large, ivy-covered house. The girl again. The Alpha Vampire on the bench. Blood cells rushing through a vein. The graveyard, dissolving into a classroom with rows upon rows of empty desks. The little girl. The alpha vamp by a pond. The dividing cell. A computer animation of the circulatory system, dissolving into a road map, where the highways pump like veins. A red circle is drawn around Aurora, Illinois. The Alpha Vampire by the pond.
The graveyard, then back to the Alpha Vampire, then the graveyard. The ivy-covered house, where the little girl is having a tea party on the lawn -- at night. She feeds her dolls blood.
The graveyard. A pulsating cell. Blood in the vein. The cell divides. Another shot of the cell dividing. The little girl is standing under a tree. Suddenly a twin flickers in beside her. She smiles, revealing a mouth full of fangs. The twin does the same. The doll with blood on her face. The Alpha Vampire on the bench, only now the twin girls are sitting on the ground next to the bench. The dividing cell. A weather vane with a bird. The alpha vamp at the pond. The twin girls play with their dolls next to the pond; they look up at the alpha vampire. He bends over and strokes one of the twins' faces with his pointed fingernail. It leaves a trail of blood on her cheek. He smiles fondly. Blood cells in the vein. The bloody doll. The graveyard. The alpha vamp and the twins; he straightens up and points. The front hallway of the house; the door opens, and the twins walk through, hand in hand. A doll's hand. One twin. The house. The Alpha Vampire reaching. A cell dividing. The graveyard. One twin. The Alpha Vampire on the bench.
Samuel and his people capture the Alpha Vampire. They subdue him using an IV of dead man's blood and attempt to interrogate him, but he is extremely resistant to pain and gives Samuel no information. After Samuel leaves the room where he is being held, the alpha is approached by Dean and Sam. He reveals to them that, though he is the first vampire, he has a mother. He recognizes Dean and he seems interested in Sam's soullessness. He also tells them that Samuel is torturing him for information about Purgatory and where it is located. Shortly after their conversation, he breaks out of his cage and almost escapes, but demons appear and take him away on Crowley's orders. It is revealed that Samuel has been working for Crowley, who will continue to question the Alpha Vampire about the location of Purgatory.
The Alpha Vampire does not appear, but is briefly mentioned when Crowley asks Sam and Dean to find another Alpha. Sam and Dean ask if the Alpha Vampire wasn't enough for him. It is possible that Crowley could get no information from him and killed him.
After learning that they need an Alpha's blood for the leviathan-killing weapon, Sam and Dean learn from Crowley that the Alpha Vampire escaped before Castiel could kill all of the monsters in Crowley's prison. Crowley gives them the Alpha's location, but when they get there, they discover that after some of his vampires were killed the Alpha fled to a hideaway he has. With the help of the Alpha's "special girl" Emily, they locate the hideaway. However, Emily, once left alone, calls the Alpha and warns him and he captures Sam and Dean when they arrive. They explain that they're there for his blood and ask for him to willingly give it to them, telling him about the leviathan food additive. They realize that the Alpha has made a deal with Dick, but point out that Dick betrayed him and has made the additive fatal to vampires. Upon hearing that Edgar has arrived and wanting to hear his side of the story, the Alpha has Sam and Dean locked up and meets with the leviathan. They discuss Sam and Dean's presence and the leviathan food additive. Edgar admits that it is poisonous to vampires and tells the Alpha that he needs all of his blood. The Alpha and Edgar fight, but despite the Alpha using borax against Edgar, he is overpowered by him and nearly killed before Sam and Dean break free and storm in, decapitating Edgar. Owing them his life and knowing they have a common enemy, the Alpha willingly gives them a glass of his blood and when its insisted upon, the kidnapped child Allan he has. They warn him to not keep Edgar's head too close to his body for too long and leave, but not before the Alpha taunts them about wanting to kill him and promising to "see you next season" which they are looking forward to.