Ectoplasm

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Ectoplasm leaking from the ear of a possessed victim.

Ectoplasm

A dense bio-energy that makes the materialisation of ghosts possible. The term ectoplasm was used in a similar sense in the 1984 film, Ghostbusters.

Pad of Definitions (2.06 No Exit), Official Website

In Supernatural, ectoplasm appears as a viscous black liquid. It is typically excreted from either the tear ducts or ears of people being possessed by a ghost or left as a residue by an extremely powerful and angry spirit.

Ectoplasm secreted by a spectre appears green instead of black. Garth is apparently able to discern ectoplasm by its taste.

Episodes

2.06 No Exit

Ectoplasm is generated by the spirit of Dr. H.H. Holmes. Sam and Dean indicate that the spirit would need to be extremely powerful (and pissed off) in order to create ectoplasm.

4.13 After School Special

Ectoplasm leaks out of the eyes and ears of the students possessed by Dirk McGregor's ghost.

7.22 There Will Be Blood

When Bobby possesses Louise, ectoplasm leaks from her eye.

The green ectoplasm left over by those possessed by Vance Collins.

8.06 Southern Comfort

The spectre of Vance Collins leaks green ectoplasm all over the place when possessing people to kill people they have a grudge against. The green ectoplasm allows them to identify Vance as a spectre from Bobby's journal.

12.03 The Foundry

When Mary is possessed by the vengeful spirit of Hugo Moriarty, ectoplasm leaks out of her eyes.

13.05 Advanced Thanatology

While possessed by Dr. Avery Meadows, Shawn Raider has ecotplasm leak from his eyes as he is forced to drill into his own head.

13.16 Scoobynatural

When the Phantom appears through a door, he leaves a large spot of ectoplasm. Later as Sam and Velma are searching the attic, Sam notices a toy chest covered in ectoplasm.