Eve

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Name Mother
Actor Darla Taylor
Dates
Location Purgatory, Earth
Occupation Mother Goddess
Episode(s) 6.12 Like A Virgin

Sam: But if you're the first, who made you?
Alpha Vampire: Well, we all have our mothers. Even me.

– Sam and the Alpha Vampire, 6.07 Family Matters

Origin

Little is known to date, other than that Mother has been in Purgatory, where the souls of the supernatural go, and that she may have been the creator of the Alphas, or first monsters.

Season 6

6.07 Family Matters

She is alluded to by the Alpha Vampire, who is being interrogated by Samuel Campbell. When Dean and Sam have a few moments alone with him, Sam asks, "But if you're the first, who made you?" The Alpha Vampire answers, "Well, we all have our mothers. Even me."

File:Mother sacrifice.jpg
The sacrifice to raise Mother from Purgatory

6.12 Like A Virgin

Dragons are abducting young women (virgins) and keeping them captive in a sewer. Dean obtains the Sword of Brunswick and he and Sam track down the dragon's lair, finding a pile of gold, and the women. They also find an ancient manuscript which speaks of freeing Mother from Purgatory. Sam and Dean kill one of the Dragon but one escapes. He meets up with another dragon who has also captured a half dozen women. They take one of the women and use an incantation to open a door to Purgatory. They throw the woman in, and she rises again giving form to Mother.

Mother in Lore

The concept of a Mother Goddess is found in cultures around the world in many forms. It is often theorised that matriarchal religions, those that worshiped a female deity, proceeded the patriarchal religions such as the Abrahamic ones (Judaism, Christianity and Islam) that worshiped a male one (God). Other religious traditions focus on the balance of the masculine and feminine within a deity or deities.

Some interpretations of European dragon myths read them as symbolising the battle of patriarchal religions to defeat the old Mother Goddess religions (represented by the dragon), with women reduced to powerless women that need to be rescued. Source.

Echidna - daughter of Gaia also known as mother nature - is a Greek goddess who is also known as the "Mother of All Monsters" because she was the source of all the monstrous creatures in Greek mythology Source.