|Powers||Can kill anything except for Lucifer and four other unknown creatures.|
Key to the Devil's Gate in Wyoming.
|Location/Owners||Men of Letters Bunker with Sam and Dean.|
|Episodes|| First Appearance: 1.20 Dead Man's Blood|
Last Appearance: 12.20 Twigs & Twine & Tasha Banes
Back in 1835, when Halley's Comet was overhead, same night those men died at the Alamo, they say Samuel Colt made a gun. A special gun. He made it for a hunter. A man like us, only on horseback. Story goes, he made thirteen bullets. This hunter used the gun a half dozen times before he disappeared, the gun along with him... Somehow Daniel got his hands on it. They say... they say this gun can kill anything.
The Colt was made by Samuel Colt in 1835. When it is used with the supernatural bullets he made specially for it, the gun can kill anything. It was the first supernatural weapon introduced in the series.
Lucifer says that there are only five things in all of creation that can't be killed by the Colt and that he is one of them, whether he meant himself personally or archangels in general was never specified. It should also be noted that while the Colt can kill (almost) anything the shot must still be a kill shot. When Sam shoots John in the leg, while he is possessed by Azazel, the demon survives, but the shot allows for John to briefly take back control of his body before Azazel escapes. The Alpha Vampire later revealed in 12.14 The Raid that the five things that the Colt cannot kill are five individual creatures rather than five specific species of monsters.
Samuel Colt also designed the locked door to keep the portal to Hell known as the Devil's Gate from opening. The Devil's Gate and the Colt are linked together, the gun serves as the key to the gate, allowing it to be opened by inserting the Colt's muzzle into the keyhole. Colt then built a railroad of iron in the shape of a pentagram, with a church at each of the points, around the Devil's Gate to further ensure that it was demon-proof.
Sam and Dean travel back in time to 1861 to use the Colt to kill a phoenix. The Colt remains in the past, possibly passing into the hands of a local saloon owner by the name of Elkins. When Samuel Colt returned to Sunrise, Wyoming to retrieve the phoenix ashes, he also left the Colt with the saloon keeper along with the special bullets for it with the hopes that it will eventually end up with Sam.
In 2006, John Winchester discovers the gun in the possession of a hunter named Daniel Elkins. Azazel makes efforts to regain it in order to open the Devil's Gate in Wyoming in order to form a demon army on Earth. While Jake Talley, who has been recruited by Azazel, opens the gate for a short time, Azazel is killed when Dean shoots him with the Colt, using the final bullet. Bobby had taken apart the Colt in an attempt to discover how it can kill anything supernatural, but was unable to figure out what makes it tick. After he started test firing it in the woods, Ruby later helps Bobby restore the Colt's supernatural killing abilities and modified it so it will take any ammunition. The Winchesters use it to kill a number of demons and vampires until it is stolen by Bela Talbot. She later trades it to Crowley to try and get out of her crossroads deal.
With the Apocalypse looming, Crowley would later return the gun to the Winchesters, telling them they can use it to kill Lucifer. However when Dean uses it on him in Carthage, Missouri, the gun is able to cause pain and disorient Lucifer after Dean shoots him in the head, but does not kill the archangel. After Lucifer regained his composure, he knocks Dean back, sending him flying into a tree where he drops the gun, leaving it behind when Castiel teleported them away. Crowley would go on to retrieve the Colt and after Lucifer's defeat, he turns the Colt along with the Lance of Michael over to one of the Princes of Hell, Ramiel as a gift while offering him the throne. He declined and convinced Crowley to take the crown in exchange for keeping himself and his remaining siblings hidden from anyone and anything, and all the while threatening to incite his wrath on Crowley if he fails to hold up his end of the bargain. Crowley accepts and leaves the Lance and the Colt with Ramiel who locks the Colt away in a supernatural wall safe with his armory of supernatural armaments.
In 2017, the British Men of Letters task Mary Winchester with regaining it from Ramiel -- without telling her what the item is, or that Ramiel is a Prince of Hell. Mick and his colleagues had been researching and studying the Colt extensively since then, however even he with all the lore on the Colt from the London Chapterhouse couldn't discover how Samuel Colt made it to kill almost anything supernatural, let alone create new bullets for it. The British Men of Letters later reveal their possession of the weapon to Sam who teaches them how to make new bullets with Bobby's recipe before using the Colt to kill the Alpha Vampire.
Following a failed attempt to kill Dagon, the Colt returns to the possession of the Winchesters who used it to kill the god Moloch. All the while, Mr. Ketch and his team had planted a surveillance system inside the Men of Letters Bunker to keep an eye on them and tried to retrieve the Colt in the process, but they eventually realize that Sam and Dean took it with them.
Castiel returned to the Bunker to talk to Sam and Dean about his temporary partnership with the angels, but it turns out to be a ruse to steal the Colt which was under Dean's pillow rather than in their safe for safe-keeping. After Joshua had tracked down Dagon through a celestial pulse caused by the Nephilim, Castiel and Kelvin went to her safe house along with Hozai and the Colt to kill both her and Kelly Kline with the remaining two bullets. He used one of the bullets to attempt to kill Dagon, but she teleported out of the way. Castiel went downstairs with every intent to kill Kelly, but he couldn't do it. After Sam and Dean had caught up with them, they explained their plan to make the Nephilim into a human by extracting its grace so both the baby and Kelly can live, but Kelly refused to let them do it because she wants her child to be born with its powers. After waiting in the Impala, Kelly had taken the keys and drove away with Castiel intending to go to Heaven's Gate, leaving the Colt with Sam and Dean. When they arrived, they discovered that Dagon had caught up with them after killing Hozai, Kelvin, and Joshua when he appeared. When Sam and Dean arrived and tried to stop Dagon from taking Kelly again, she attacked Dean and took the Colt from him. Dagon used her power to heat up and melt the barrel of the Colt, causing the muzzle to fall off and severely damaging the gun. Castiel is later able to kill Dagon without the Colt using the powers of the Nephilim; Jack Kline.
Sam retrieves the pieces of the damaged Colt and takes them back to the Bunker where it's shown that the heat has deformed parts of the gun's mechanisms. Sam promises to attempt to repair the legendary gun, but he is unsure if he can.
Invented by Samuel Colt, a gun named after its inventor “Colt”, and after its revolving cylinder. Before the Colt revolver, only one and two-barrel flintlock pistols had been invented for hand-held use. Colt revolvers were all based on cap-and-ball technology until the Smith and Wesson license on the bored-through cylinder (bought from Rollin White) expired around 1869.
The Colt used in Supernatural has some special "features":
Latin Inscription: Non timebo mala translates to "I will fear no evil" from Latin, and is a reference to Psalm 23 and the quote "For though I should walk in the midst of the shadow of death, I will fear no evil, for thou art with me." It has inspired many Fan Tattoos. The font used is Plakat Fraktur.
Pentagram: See Pentagram
Make and Model of The Colt: The Colt has the appearance of an 1836 Colt Paterson Pietta Paterson Super Deluxe. In reality, the Colt Paterson, which is the first Revolver ever built, is surprisingly difficult to load. It can be assumed that the gun itself was modified in 3.04 Sin City when Bobby agreed to let Ruby restore its supernatural killing abilities which would explain how it can be easily reloaded without undergoing the painstakingly long process of deconstructing it's barrel. See: History of the Colt Paterson.
Bullets: The 13 bullets made by Samuel Colt are engraved with numbers. Whenever The Colt fires a bullet, a cross within a circle along with a few vestiges of ancient scripture appear on the tip of it and burns orange-red right before it hits anything. It was first noticed in 2.22 All Hell Breaks Loose: Part Two when Dean uses the final original bullet in the chamber to kill Azazel. Whenever the Colt was used in Season 12, the cross can be seen in 12.14 The Raid when the Alpha Vampire was killed by Sam and 12.17 The British Invasion when Eileen Leahy intended to shoot Dagon, but hits Renny Rawlings when she teleports out of the way. The cross appears to resemble a Cross Potent, the Sun Cross, or a Ringed Cross.
Ruby was able to build more bullets for the Colt in 3.04 Sin City, and later in 12.14 The Raid, Sam relates that Bobby had learned the method from her and passed it on to him. The process requires holy oil, sage and myrrh. They are mixed to make a tincture and used to coat silver bullets and the following spell must be recited to mimic the original etchings: "Signum est imitandum. Signum est imitandum."
Chronological Appearances and Uses
Use of the Colt
How Samuel Colt's original bullets are used
- 6.18 Frontierland - Samuel Colt, in 1861, shoots and kills two demons. Colt gives Sam the gun to give to Dean, who shoots and kills the phoenix.
- 1.20 Dead Man's Blood - The Colt is introduced with 5 (out of 13) remaining bullets. John Winchester shoots Luther, the vampire to save Sam's life.
- 1.21 Salvation - Sam shoots at the Yellow-Eyed Demon in Rose's nursery.
- 1.22 Devil's Trap - Dean shoots the demon Tom to save Sam's life. Sam later shoots John in the thigh while he is possessed by the Yellow-Eyed Demon.
- 2.22 All Hell Breaks Loose: Part Two - Dean kills Azazel with a shot in the heart.
- 2.22 All Hell Breaks Loose: Part Two: The Colt is used by Jake as a key to open the Devil's Gate in Wyoming.
- 3.04 Sin City: Ruby helps Bobby rebuild the Colt so it will use any ammunition. Sam then uses it to kill Father Gil and Casey. This also marks the first time the Colt bullets now give off an orange-red hue when they kill a demon much like the bullet's effects with Azazel, but now it's for every demon Sam and Dean use the Colt on.
- 3.05 Bedtime Stories: Sam uses the Colt to kill a crossroads demon.
- 3.07 Fresh Blood: Dean shoots a vampire with it. This is the first time after Ruby helps Bobby make more bullets for the Colt, as well as the last time (so far), that we've seen the blue-colored hue being used after Dean kills the vampire. It can be suggested that the blue hue is only used against monsters now.
- 3.09 Malleus Maleficarum: First Dean threatens to shoot Ruby with it, but Sam stops him. Later Sam tries to shoot Tammi with the Colt, but she stops the bullet in mid-air.
- 3.10 Dream a Little Dream of Me: Bela steals the Colt.
- 3.15 Time Is on My Side: Bela gave the Colt to demons to try and get her way out of her crossroads deal, but it doesn't go as planned for her.
- 5.09 The Real Ghostbusters: Becky tells Sam that in Chuck's book of Time Is on My Side, Bela gave the Colt to a crossroads demon named Crowley instead of Lilith.
- 5.10 Abandon All Hope...: Crowley used it to kill two of his "hench-demons" and gives the Colt to the Winchesters along with some more bullets. During the hunt that follows, Dean shoots and presumably kills a hellhound with the Colt. The boys then track down Lucifer and Dean shoots him straight through the head but Lucifer is unharmed and he flings Dean towards the trees and the Colt is last seen in Dean's hand after he lands. He also mentions he is one of five things in all of Creation that the Colt cannot kill. 
- 12.14 The Raid: Sam uses it to kill the Alpha Vampire with a shot through the head.
- 12.17 The British Invasion: Eileen Leahy accidentally kills Renny Rawlings when Dagon vanishes as she takes her shot.
- 12.18 The Memory Remains: Sam uses it to kill the god Moloch.
- 12.19 The Future: Castiel attempted to kill Dagon with the Colt, but she teleported out of the way, while the bullet struck the wall.
When Daniel Elkins is killed, he leaves a note for John Winchester saying that he had "it" (along with the five remaining bullets) in his possession. John is interested in retrieving it since the Colt can supposedly kill anything supernatural, it could possibly be used to kill the Yellow-Eyed Demon and avenge Mary's death. After retrieving the Colt from the vampires who killed Elkins and looted his property, John uses it to kill the vampire pack leader, Luther, proving that the gun is the one he's been searching for, as an ordinary revolver would not have killed a vampire.
Dean uses the third remaining bullet to kill the demon Tom, after he and Sam rescue John. Their father, possessed by the Yellow-Eyed Demon, begs Sam to shoot him, but he cannot kill his father and uses the fourth bullet to shoot John in the leg, the hesitation to kill the demon and John with him, allowing it to flee.
The Yellow-Eyed Demon gives the Colt to Jake Talley in order to open the Devil's Gate in Wyoming. After making his way to Fossil Butte Cemetery, Jake succeeds in opening the gate by threatening Ellen's life, but after Sam kills him, Dean retrieves the Colt from the crypt door. The Yellow-Eyed Demon shows up and takes the Colt from Dean, but after John's spirit distracts the demon by ripping him out of his meatsuit, Dean grabs the Colt back and shoots Azazel in the heart with the last bullet just as he returns to his host, finally killing him. Bobby and Ellen were later able to seal the devil's gate before any more demons or spirits could escape.
Bobby is seen working on the Colt.
Bobby is trying to figure out how the Colt works when Ruby arrives and offers to help return its demon killing powers. Bobby later shoots at the possessed Father Gil with it -– and misses. Sam eventually uses it to kill Father Gil and Casey.
The Crossroads Demon outside of Lloyd's Bar recognizes that the Colt has been modified, stating that it was not "the original Colt." She realizes that Ruby was the one who modified it. Sam eventually uses the Colt to kill the crossroads demon to try to get Dean out of his contract.
Dean discovers that Sam tried to get him out of his contract after he notices that a bullet is missing from the Colt. After confronting Sam, he tells Dean that he's not going to apologize for saving his life.
When Bela calls the Winchesters to taunt them about stealing the Colt, it is seen on the seat next to her sitting on a towel. Later, Ruby is annoyed to learn that the Colt is gone when they have to face off against an army of around thirty demons.
When Dean goes back to 1973 he "borrows" the Colt from a young Daniel Elkins, telling him the gun will be in Lawrence, Kansas. He has two opportunities to shoot Azazel, but the demon flees before he gets the chance to kill him.
Castiel tells Dean that he has learned the demons have not destroyed the gun. In 2014, Future Dean finally retrieves the Colt from the demons and tries to kill Lucifer with it, but fails and is killed.
Becky tells Sam that in the Supernatural book written by Chuck Shurley which tells the events of 3.15 Time Is on My Side, Bela gave the Colt not to Lilith but to Crowley; her right hand demon and possible lover.
After using it to kill two of his hench-demons, Crowley gives the Colt and some more bullets to the Winchesters to kill Lucifer who is in Carthage, Missouri. Dean uses it against the hellhounds, and either wounds, or kills one, but is unable to stop them all. Dean shoots Lucifer in the head at point-blank range, telling him to "suck it." Lucifer falls and appears to be dead at first, but he gets up in pain, the bullet wound in his forehead heals, and he punches Dean into a tree, dropping the Colt in the process. Lucifer goes on to explain to Sam that he is one of five things in all of creation that the gun can't kill. It was later revealed that when Castiel teleported Sam and Dean out of the battlefield, the Colt was left behind and was retrieved by Crowley while Lucifer was releasing Death from his prison.
Sam was able to take Dean and Bobby to the library in the Campbell Compound to look through Samuel's lore books and find a way to kill Eve. Bobby was able to find out that the ashes of a phoenix can kill Eve and Dean recovers Samuel Colt's Journal and discovers that in March 5, 1861 the Colt was used to kill a phoenix in Sunrise, Wyoming. They were able to summon Castiel so they can travel back to 1861, but they only have 24 hours before he brings them back. Samuel Colt is retired from hunting, and completing the railway devil's trap to protect the Devil's Gate. Two demons enter his home looking for the gun, and he shoots them with the Colt. When Sam arrives and asks for his assistance, he refuses, but eventually gives Sam the Colt. Dean uses the Colt to kill Elias Finch — the phoenix.
However, Dean drops the Colt just before he and Sam are transported back to the present day. It is possible that the Colt was retrieved by the saloon owner Elkins. In the present day, the Colt was originally in the possession of Daniel Elkins and its possible that the Elkins of 1861 was an ancestor who passed it down to him. It was stated in Colt's Journal that he knew the saloon keeper personally. Since there were two bullets left in the chamber at most after it was used to kill two demons and a phoenix, and the fact that Daniel Elkins was loading five bullets into the Colt when it was first introduced in 1.20 Dead Man's Blood, it's implied that while Samuel Colt returned to Sunrise, Wyoming to retrieve the phoenix ashes, he also gave the Colt to the saloon keeper Elkins along with the special bullets with the hopes that it will eventually end up with Sam.
In a flashback to 2011 following Lucifer's defeat, Crowley turns the Colt along with the Lance of Michael over to the Prince of Hell Ramiel as a gift to take Hell's Throne, but Ramiel refuses and offers Crowley the throne instead in exchange for maintaining the solitude of his fellow siblings. After their deal, Ramiel stashes the Colt and the Lance into his supernatural armory.
In the present, Mary Winchester is sent by the British Men of Letters to steal the Colt from Ramiel. While she succeeds with the help of her sons, Castiel and the hunter Wally, Wally is killed and Castiel is gravely injured as Ramiel chases them to retrieve the Colt. Ramiel offers the Winchesters their lives if they give him back what they stole, but Sam and Dean have no idea what he's talking about. After a fight, Ramiel is killed by Sam with the Lance of Michael, leaving Sam and Dean unaware of what Mary did.
After killing Ramiel, Mary meets with Mr. Ketch and threatens to kill him and the British Men of Letters in general after what happened to Wally and what almost happened to Castiel, because they unknowingly sent them after a Prince of Hell. After a moment of silence, Mr. Ketch admits that they made a mistake and apologizes. She then hands over the Colt, telling him that her father used to tell her stories about the gun. Crowley also sent an army of his demons to Ramiel's house to retrieve the gun and panics when they fail to locate the Colt while Lucifer taunts him about it in his "doghouse."
After learning that the Alpha Vampire is attacking, Mary Winchester has the British Men of Letters reveal to Sam that they possess the Colt, the only weapon they have that can kill the Alpha Vampire. While the British Men of Letters have no bullets and don't know how to make any, Sam is able to give them Bobby Singer's recipe and they create new bullets shortly before the Alpha Vampire enters. Sam bluffs the Alpha Vampire with the empty Colt, with the Alpha Vampire claiming to be one of the five things the gun can't kill. Sam tries to negotiate a deal with the Alpha Vampire before Mary attacks him and Mick Davies barreled after Sam in a ruse to get him a bullet for the Colt so he can load it. Once Sam reveals this, the Alpha Vampire calls him a "clever, clever boy" moments before Sam shoots him through the head with the Colt. As the Colt's power works on him, the Alpha Vampire chuckles briefly before dropping to the ground, dead.
While going after the Prince of Hell Dagon, Mick Davies brings the Winchesters the Colt to use against the powerful demon. In the brief fight that follows, Eileen Leahy grabs the Colt off the ground and shoots at Dagon. Dagon teleports away just before the bullet hits her. Instead, the shot strikes Renny Rawlings in the heart, killing him. While leaving the scene, Eileen holds onto the Colt rather than returning it to Mick.
After Eileen leaves, Sam reveals to Dean that she has left them the Colt. Dean tests the weapon, clearly excited to have ownership of the legendary gun once more.
Dean arms himself with the Colt in lieu of his regular pistol. While hunting Moloch, Pete Garfinkle steals the Colt and tries to use it to kill Sheriff Barrett Bishop, Jr. before he himself is killed by Sam. Sam then uses the Colt to kill Moloch with a shot through the chest, causing Moloch to dissolve into a puddle.
While in the Bunker, Mr. Ketch orders his team to search for the Colt which Mick had let the Winchesters take. The team is unable to find the Colt and Mr. Ketch is annoyed to realize that the Winchesters must have it with them.
Castiel returns to the Bunker from Heaven. He steals the Colt, from under Dean's pillow where Dean has kept it rather than locking it in the safe. Castiel along with some other angels find Dagon and Kelly Kline, entering the home Castiel misses when he tries to kill Dagon, and then is unable to kill Kelly when faced with the task. After his truck breaks down, Sam and Dean find Castiel and Kelly, and Dean takes possession of the Colt. When Kelly and Castiel take off and try to return to Heaven, Dagon appears, waiting for them. The Winchesters arrive and Dean tries to kill her with the Colt, but Dagon takes the weapon from him, and uses her power to melt the gun's barrel, deforming it and causing the Colt's muzzle to fall off. Castiel is later able to use the Nephilim's powers to kill Dagon without the Colt through spontaneous combustion.
In the aftermath of Castiel disappearing with Kelly Kline, Dean asks Sam if he is able to fix the damaged Colt, which is now in two pieces, who is hopeful, but uncertain.
Legend of the Colt
Samuel Colt (1814-1862) was the inventor of the Colt revolver, and founder of the Colt Firearms company. His huge achievement was for inventing a functional design for a gun that could shoot multiple times before having to be reloaded. He was highly influential in the munitions industry and was a noted inventor. See also:
It should be noted that Samuel Colt spent 1835 in England and France, securing the first patent on his revolving-breech pistol. He returned home and obtained an American patent in 1836.
The Battle of the Alamo was a 19th-century battle between the Republic of Mexico and the rebel Texian forces during the latter's fight for independence - the Texas Revolution. It took place at the Alamo mission in San Antonio, Texas (then known as "San Antonio de Béxar") in February and March of 1836.
History of Conflicts
Although most people automatically think of the famous battle that occurred in 1836 when the Alamo is mentioned there is a history of minor battles that occurred at the Alamo. The Alamo has a long and bloody history beginning in the mid to late 1700's.
In October of 1835 one of many battles occurred between residents of Gonzales and the Mexican troops that occupied the Alamo at the time. This skirmish led to Santa Anna sending in troops under Martin Perfecto de Cós to San Antonio. This was only one of many smaller battles over the course of October, November, and December of 1835 that eventually led to the famous battle that occurred in February and March of 1836.
For more information about the history/timeline of the Alamo check out the Daughters of the Republic of Texas website. They include a detailed history of the Alamo and all its battles.
The battle in 1835 is usually referred to as the Siege of Béxar, was the first battle for the occupation of the Alamo, in which the Texians took possession from the Mexican Governmental troops. It took place between October 12 and December 11th, 1835. On December 7th, the Texians had all of their artillery in place and began their assault on the Alamo. The flag of surrender was sent out from the Alamo to the Texian army on December 9th. So a good guess as to the exact 'night those men died' would be December 8th, 1835. (sources Siege of Béxar (Bejar) - Wikipedia Siege of Béxar (Bejar) - Wikipedia and Siege & Battle of Béxar from the Dairy of Samuel Maverick)
Comet Halley ... more generally known as Halley's Comet after Edmond Halley, is a comet that can be seen every 75-76 years. It is the most famous of all periodic comets, even though in every century many comets appear brighter and more spectacular. Halley's comet last appeared in the inner Solar System in 1986, and will next appear in the summer of 2061." (Source: Wikipedia)
In 1835, it achieved perihelion on 16 November, but would have been visible from Earth for several weeks before and after.
Arizona claims Colt
- Arizona has adopted the Colt as its state firearm (April, 2011)
The Texas Paterson 1836
The first type of production handgun made by the real Samuel Colt is a Texas Paterson 1836. Supernatural Prop Master Chris Cooper found a replica of the gun at a Las Vegas gun show. The original revolver was a powder and cap gun, but the Supernatural prop department made a new revolver piece that could take cartridges.
Although not obvious to anyone not familiar with the Paterson model, "the Colt" does in fact, have a trigger. While the Colt Paterson Revolvers in general, as well as "the Colt" do not have trigger guards, the trigger for these revolvers are inset flush into base of the frame, and drop down automatically when the hammer is cocked. The trigger can be clearly seen behind Bobby's finger when he is test-firing it in the scene where Ruby first appears to help him modify it to restore its demon killing abilities. The trigger and the hammer can also be seen up close when Sam kills the Alpha Vampire with The Colt in 12.14 The Raid.
Speculation by Fans
The legend of the Colt has brought up a lot of speculation between the fans due to some inconsistencies with its history.
The Five Things
Lucifer says that there are only five things in all of creation that can't be killed by the Colt and that he is one of them. There's no definite answer whether or not Eve can be killed by the Colt. Death could be more powerful than the archangels since in the end he would reap God and he told Dean that he existed at least as long as God, possibly more, but neither one of them can remember exactly how long anymore. The other 3 Horsemen: Famine, Pestilence and War: are immortal, but powerless without their rings unlike their brother Death who is the ultimate reaper. The only way the other three Horsemen were stopped was by cutting off their rings, but they did not die. Therefore, there's reason to believe the Colt can't kill them. However, there might be a reason to separate Death and the Horsemen since they are on different power levels. If this is true, then Eve can be killed by the Colt, since the Horsemen are older and bump her off the list. Since Death is the only Horseman of the Apocalypse who doesn't actually need his ring, it stands to reason that Death is immune to the Colt while the other three Horsemen are not. Another question is that could the Colt kill Cain? He was the one with the First Blade and the Mark of Cain which evidently renders the bearer immortal. If the user dies, it comes back as a Knight of Hell. The First Blade killed Cain. If Cain cannot be killed with the Colt, then it could be because he was either Adam and Eve's firstborn son or because he was the holder of the Mark of Cain, as he was made into a Knight after the Princes of Hell were made, who are susceptible to the Colt like Azazel. Since the Princes existed before him, it proves that Cain and the other Knights of Hell can be killed by the Colt. The Alpha Vampire told Sam that "there are five creatures on this Earth that gun cannot kill" and tried to pass of that he was one of them, but Sam doesn't believe him and eventually kills him. It confirms that the five things that the Colt cannot kill are five individual creatures rather than five specific species of monsters. Since Lucifer was unaffected by the Colt, it points out that Michael can't be killed by it either and it was previously stated that both Michael and Lucifer appear to be on different power levels than Gabriel and Raphael. It raises the question if Gabriel and Raphael are susceptible to the Colt, but since both archangels were trapped in a ring of holy fire which had to be extinguished by water to release them and Lucifer was trapped in a ring of holy fire that extinguished itself, despite the warding used to trap him and the fact that Michael was molotov cocktailed by Castiel with a flaming bottle filled with holy fire and survived, it shows that Gabriel and Raphael may be somewhat weaker than Michael and Lucifer, presenting the possibility that they could be killed by the Colt.